Extracellular Vesicle Encapsulated MicroRNAs in Patients with Type 2 Diabetes Are Affected by Metformin Treatment
Year: 2019
Type: article
Abstract: Recently, microRNAs (miRNAs) in circulating extracellular vesicles (EVs), have emerged as a source of potential biomarkers for various pathophysiological conditions, including metabolic disorders such as diabetes. Type 2 diabetes mellitus (T2DM), is the most prevalent form of diabetes in the USA, with 30 million diagnosed patients. Identifying miRNA biomarkers that can be used to assess response to glucose lowering treatments would be useful. Using patient plasma samples from a subset of the Danish Metagenomics of the Human Intestinal Tract (MetaHIT) cohort, we characterized miRNAs from whole plasma, plasma-derived EVs, and EV-depleted plasma by small RNA-sequencing to identify T2DM associated miRNAs. We identified several miRNAs that exhibited concentration changes between controls and non-metformin treated T2DM patients and we validated a subset of these by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The results showed that the concentrations of many T2DM-affected miRNAs in EV (but not in whole or EV-depleted plasma) decreased to levels close to those of healthy controls following metformin treatment. Among other potential uses of these differentially expressed miRNAs, some might be useful in assessing the response to metformin in T2DM patients.
Source: Journal of Clinical Medicine
DOI: 10.3390/jcm8050617
Authors: Vikas Ghai, Taek-Kyun Kim, Alton Etheridge, Trine Nielsen, Torben Hansen, Oluf Pedersen, David Galas, Kai Wang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,824
Awards: AN APPROACH USING THE CHANGES OF CIRCULATING RNA SPECTRUM AND MICROBIOME SIGNATURES TO DETECT AND ASSESS THE HEALTH IMPACT OF CHEMICAL WARFARE AGENT, G2768705171, G3504303860, G4504918650, miRNAs, Whole Diet, and Coronary Heart Disease, Accelerated emphysema in HIV-infected smokers: the role of extracellular vesicles, CONTINUATION STUDY: A SYSTEMS APPROACH TO UNDERSTANDING POST-TRAUMATIC STRESS DISORDER, G9000013491, G9000316288
OpenAlex ID: W2943887805