Multiplane Calcium Imaging Reveals Disrupted Development of Network Topology in Zebrafishpcdh19Mutants
Year: 2019
Type: article
Abstract: Functional brain networks self-assemble during development, although the molecular basis of network assembly is poorly understood. Protocadherin-19 (pcdh19) is a homophilic cell adhesion molecule that is linked to neurodevelopmental disorders, and influences multiple cellular and developmental events in zebrafish. Although loss ofPCDH19in humans and model organisms leads to functional deficits, the underlying network defects remain unknown. Here, we employ multiplane, resonant-scanningin vivotwo-photon calcium imaging of developing zebrafish, and use graph theory to characterize the development of resting state functional networks in both wild-type andpcdh19mutant larvae. We find that the brain networks ofpcdh19mutants display enhanced clustering and an altered developmental trajectory of network assembly. Our results show that functional imaging and network analysis in zebrafish larvae is an effective approach for characterizing the developmental impact of lesions in genes of clinical interest.
Source: eNeuro
Authors: Sarah E.W. Light, James D. Jontes
Institution: The Ohio State University
Language: English
Subfield: Cognitive Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,925
Funder: National Eye Institute
OpenAlex ID: W2944075648