Work

The effect of saturated and unsaturated fatty acids on the production of outer membrane vesicles from Bacteroides fragilis and Bacteroides thetaiotaomicron.

Year: 2019

Type: article

Abstract: AIM: spp. BACKGROUND: . METHODS: were grown on BHI broth with and without palmitic and palmitoleic acids as saturated and unsaturated fatty acids, respectively. OMVs were extracted using multiple centrifugation and tris-ethylene diamine tetra acetic acid (EDTA)-Sodium deoxy cholate buffers. Physicochemical properties of OMVs were detected by electron microscopy (SEM), Bradford Coomassie brilliant blue assay and SDS-PAGE. Data were analyzed with One-way ANOVA using SPSS. RESULTS: OMVs at low concentration. CONCLUSION: OMVs was affected by palmitoleic acid in a dose dependent manner.

Source: Gastroenterology and hepatology from bed to bench.

Authors: Zahra Sadat Mirjafari Tafti, Arfa Moshiri, Fateme Ettehad Marvasti, Samira Tarashi, Seyedeh Fatemeh Sadati Khalili, Atiyyeh Motahhary, Abolfazl Fateh, Farzam Vaziri, Sara Ahmadi Badi, Seyed Davar Siadat

Institutions: Pasteur Institute of Iran, Istituto Giannina Gaslini, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University, Shahid Beheshti University of Medical Sciences

Language: English

Topic: Bacterial Infections and Vaccines

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: diamond

Funders: Pasteur Institute of Iran, National Institute for Medical Research Development

Award: G767424722

OpenAlex ID: W2944199151

FWCI: 1.673

Cites: 46

Cited by: 17

Related to: 10