Year: 2019
Type: article
Abstract: ), influenced cell-to-cell variability. The experiments showed robust growth of populations at intermediate concentrations and cell-to-cell variability was higher at low and high carbon concentrations, among an isogenic population. Single-cell growth at extremely limiting conditions led not only to significant variability of division times, but also to an increased number of cells that did not pursue growth. Overall, the results demonstrate that cellular behaviour shows robust, Monod-like growth, with significant cell-to-cell heterogeneity at extreme limiting conditions, resembling natural habitats. Due to this significant influence of the environment on cellular physiology, more carefulness needs to be given future microfluidic single-cell experiments. Consequently, our results lay the foundation for the re-interpretation and design of workflows for future experiments aiming at an improved understanding of cell growth mechanisms.
Source: RSC Advances
DOI: 10.1039/c9ra02454a
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $937
Awards: G1568319151, e:Bio - Modul II - Verbundprojekt: 0,6Plus - Verbesserung grundlegender Wachstumseigenschaften von C. glutamicum zur Verbreiterung der industriellen Anwendbarkeit - Teilprojekt C, Biotechnologie2020+ Strukturvorhaben: Helmholtz Research Network - Molecular Interaction Engineering, Teilprojekt des FZJ, G6749248756
OpenAlex ID: W2944475826