The two froms of neurotrophins and their neuroprotective effect on inner ear specific cells
Year: 2019
Type: conference-paper
Abstract: Introduction: Numerous in vitro and in vivo studies show that neurotrophins protect the cells of the inner ear from degeneration. Therefore, the administration of neurotrophins is a promising pharmacological approach in cochlear implant research. Neurotrophins, however, are highly increased and released endogenously, initially as a pro-form, which is then converted into the mature form by proteolytic cleavage. Generally, brain-derived neurotrophic factor (BDNF) is administered in its mature form. The aim of this study is to investigate whether pro-BDNF, despite its apoptosis-inducing properties, can be administered to maintain endogenous homeostasis between pro-BDNF and BDNF. Material: First, the expression of both BDNF forms and their receptors in the murine cochlea was analyzed by immunocytochemical antibody staining. Additionally, dissociated spiral ganglion cells (SGC) from neonatal rats (P3 – 5) were incubated with BDNF and pro-BDNF alone as well as in combination and the survival rates were determined. Results: Both forms and their corresponding receptors are expressed in the organ of Corti and in the spiral ganglion of adult cochleae. Treatment with pro-BDNF alone has no neuroprotective effect but also no toxic effect on SGC. When administered in combination with BDNF, pro-BDNF increases the survival rate of SGC (both forms are almost in equilibrium) and reduces the survival rate (imbalance between the two forms). Conclusion: These results suggest that a balanced ratio of pro-BDNF and mature BDNF matches more precisely the physiological conditions in the cochlea and may even mediate the neuroprotective effect. Publication History Publication Date: 23 April 2019 (online) © 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/). Georg Thieme Verlag KG Stuttgart · New York
Source: Laryngo-Rhino-Otologie
Authors: J Schulze, H Staecker, T Lenarz, D Wedekind, D Werner, A Warnecke
Language: English
Subfield: Sensory Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2945900984