Aggregability of Mammalian and Avian Thrombocytes with Special Respect to the Effect of Various Antithymocyte and Antilymphocyte Globulins
Year: 1977
Type: conference-paper
Abstract: Although interest in platelets is rapidly growing, systematic comparative studies on platelet aggregation are still limited. Here, the aggregation in citrated platelet (thrombocyte)-rich plasmas (PRP) from 18 mammalian (man; chimpanzee; baboon; cynomologus, rhesus, pigtailed, stumptailed, and squirrel monkeys; dog; cat; cow; goat; sheep; horse; rabbit; rat; hamster; guinea pig) and one avian species (chicken) was measured by Bom’s turbidimetric method with agents at final concentrations as given below. Despite the quantitative differences, ADP (5 μM), acid-soluble calf skin collagen (120 μg/ml) or bovine thrombin (0.5 U/ml) aggregated in all mammalian PRPs. In contrast, epinephrine (25 μg/ml) caused aggregation in canine, feline and some primate PRPs, whereas ristocentin (1.5 mg/ml) did in PRPs of certain primates. Chicken thrombocytes were slightly reactive only to collagen and a 4-fold dose of thrombin. Because platelets share antigens with lymphocytes, it was not surprising that all unabsorbed antithy-mocyte (horse anti-human; rabbit anti-rat, -mouse, -chicken ATGs) and antilymphocyte globulins (rabbit anti-rhesus, -rat, -mouse, -hamster, -guinea pig ALGs) tested exhibited platelet aggregating activity. The cross-reactivity of ATGs or ALGs always followed the phylogenetic order qualitatively, but the quantitative relations (extent of aggregation) frequently differed from that pattern. As an explanation, variations in susceptibility to aggregation among various species can be suggested. In parallel studies, goat antirhesus and rabbit antibaboon platelet sera revealed wide cross-reactions between mammalian species, however, like anti-mammal i an ATGs or ALGs, not for the chicken.
Source: Thrombosis and Haemostasis
Authors: E.A. Suba, G. Csákó
Institution: Naval Medical Research Command
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2946152907