Work

PRECOG: PREdicting COupling probabilities of G-protein coupled receptors

Year: 2019

Type: article

Abstract: G-protein coupled receptors (GPCRs) control multiple physiological states by transducing a multitude of extracellular stimuli into the cell via coupling to intra-cellular heterotrimeric G-proteins. Deciphering which G-proteins couple to each of the hundreds of GPCRs present in a typical eukaryotic organism is therefore critical to understand signalling. Here, we present PRECOG (precog.russelllab.org): a web-server for predicting GPCR coupling, which allows users to: (i) predict coupling probabilities for GPCRs to individual G-proteins instead of subfamilies; (ii) visually inspect the protein sequence and structural features that are responsible for a particular coupling; (iii) suggest mutations to rationally design artificial GPCRs with new coupling properties based on predetermined coupling features.

Source: Nucleic Acids Research

DOI: 10.1093/nar/gkz392

Authors: Gurdeep Singh, Asuka Inoue, J Silvio Gutkind, Robert B Russell, Francesco Raimondi

Institutions: Heidelberg University, Tohoku University, University of California San Diego, Moores Cancer Center

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,992

Funders: Japan Agency for Medical Research and Development, Deutsche Forschungsgemeinschaft, Japan Society for the Promotion of Science