Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology.
Year: 2019
Type: article
Abstract: BACKGROUND: Expression of heterologous proteins at large scale is often a challenging job due to plasmid instability, accumulation of acetate and oxidative damage in bioreactors. Therefore, it is necessary to optimize parameters influencing cell growth and expression of recombinant protein. METHODS: ., temperature, shaking speed and pH) and their interactions with cellular growth and protein production. The obtained data were analyzed by Design Expert software. RESULTS: . CONCLUSION: ., pH, temperature and oxygen supply) in protein expression at large scale and confirm the need for optimization. The proposed strategy here can also be applied to experimental set-up of optimization for fermentation of other proteins.
Source: Europe PMC (PubMed Central)
Institution: Isfahan University of Medical Sciences
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2947525246