Optimization and Validation of a Pathogenesis Survival Assay with the C. elegans Sterile Mutant Fer-1
Year: 2012
Type: article
Abstract: An infertile C. elegans mutant fer-1 was identified as a suitable substitute for wild type in evaluating the pathogenesis of C. albicans using a survival assay. The response of fer-1 mutant worms infected with avirulent C. albicans mutants or treated with fluconazole is statistically indistinguishable from the wildtype counterpart. The lifespan of uninfected fer-1 worms is marginally yet reproducibly shorter than wildtype, but the lack of progeny in sterile mutants makes them an ideal choice for otherwise labor-intensive lifespan analyses.
Source: Digital WPI
Authors: Stephanie Post, Tracy Lynn Sears
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2947687202