Work

Anti-inflammatory effect of aged black garlic on 12-O-tetradecanoylphorbol-13-acetate-induced dermatitis in mice

Year: 2019

Type: article

Abstract: BACKGROUND/OBJECTIVES:Although aged black garlic has various biological activities such as anti-allergy, anti-inflammation and neuroprotection, effect of aged black garlic on chemically contact dermatitis is unclarified.MATERIALS/METHODS: To evaluate anti-dermatitic activity of aged black garlic extract, we investigated effects of a fraction of aged black garlic extract (BG10) on both in vivo and in vitro.RESULTS: BG10 almost inhibited formation of nitric monoxide and interleukin-6 (IL-6; IC50, 7.07 μg/mL) at 25 μg/mL, and dose-dependently reduced production of tumor necrosis factor-α (TNF-α; IC50, 52.07 μg/mL) and prostaglandin E2 (IC50, 38.46 μg/mL) in lipopolysaccharide-stimulated RAW264.7 cells.In addition, BG10 significantly inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2 and nuclear NF-κB, and improved that of cytosolic levels of NF-κB and IκBα in the cells.Consistent with in vitro studies, BG10 (0.5 mg/mL) not only reduced ear edema but also suppressed the formation of IL-6 and TNF-α induced by 12-O-tetradecanoylphorbol-13-acetate in ear tissues of mice.CONCLUSIONS: These findings suggest BG10 has anti-dermatitic activity through inhibiting activation of macrophages.Therefore, such effects of BG10 may provide information for the application of aged black garlic for prevention and therapy of contact dermatitis.

Source: Nutrition Research and Practice

DOI: 10.4162/nrp.2019.13.3.189

Authors: Bo Ram You, Jae-Myung Yoo, Seong Yeon Baek, Mee Ree Kim

Institutions: Chungnam National University, Korea Institute of Oriental Medicine

Language: English

Topic: Garlic and Onion Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Chungnam National University