Work

Manipulating Immune Activation of Macrophages by Tuning the Oligonucleotide Composition of Gold Nanoparticles

Year: 2019

Type: article

Abstract: activation of macrophages. Nanoconstructs with similar ligand densities but different oligonucleotide compositions (from 0% to 100% immune-active cytosine-phosphate-guanine, CpG) were compared. Maximum immunostimulation was achieved with CpG content as low as 5% (with total oligonucleotide surface coverage remaining constant), correlating to high levels of antitumor cytokine release and low levels of cancer-promoting ones. Independent of CpG content, gold nanoparticles with low oligonucleotide densities exhibit poor cellular uptake, leading to insignificant immunostimulation and cytokine release. By identifying effects of ligand shell composition on macrophage activation, we can inform the design rules of therapeutic nanoconstructs to achieve specific immune responses.

Source: Bioconjugate Chemistry

DOI: 10.1021/acs.bioconjchem.9b00316

Authors: Roger M. Pallares, Priscilla Choo, Lisa E. Cole, Chad A. Mirkin, Andrew Lee, Teri W. Odom

Institutions: Northwestern University, International Institute for Nanotechnology

Language: English

Topic: Immunotherapy and Immune Responses

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Prostate Cancer Foundation, Movember Foundation, National Cancer Institute