Work

Novel tools for constructing markerless deletions in Clostridium saccharobutylicum NCP262

Year: 2019

Type: dissertation

Abstract: We developed two different markerless deletion systems for C. saccharobutylicum: One uses conjugation as a delivery method for a suicide vector and the codBA genes and 5-fluorocytosine for counterselection. The second one uses electroporation as a delivery method for a suicide vector and the upp gene and 5-fluorouracil for counterselection. They represent valuable tools for the genetic and metabolic engineering of this industrially important solvent producing organism.

Source: mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich)

Author: Ching-Ning Huang

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: European Commission, FP7 Food, Agriculture and Fisheries, Biotechnology

Awards: A Clostridal Biology Network to Facilitate European-wide Medical Countermeasures and Commercial Exploitation, G7582053920, Valorisation of biorefinery by-products leading to closed loop systems with improved economic and environmental performance, G838461549, G994389065

OpenAlex ID: W2948910905

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