Work

Toxoplasma Does Not Secrete the GRA16 and GRA24 Effectors Beyond the Parasitophorous Vacuole Membrane of Tissue Cysts

Year: 2018

Type: article

Abstract: After invasion, Toxoplasma resides in a parasitophorous vacuole (PV) that is surrounded by the PV membrane (PVM). Once inside the PV, tachyzoites secrete dense granule proteins (GRAs) of which some, such as GRA16 and GRA24, are transported beyond the PVM likely via a putative translocon. However, once tachyzoites convert into bradyzoites within cysts, it is not known if secreted GRAs can traffic beyond the cyst wall membrane. We used the tetracycline inducible system to drive expression of HA epitope tagged GRA16 and GRA24 after inducing stage conversion and show that these proteins are not secreted beyond the cyst wall membrane.

Source: Frontiers in Cellular and Infection Microbiology

DOI: 10.3389/fcimb.2018.00366

Authors: Shruthi Krishnamurthy, Jeroen PJ Saeij

Institution: University of California, Davis

Language: English

Topic: Toxoplasma gondii Research Studies

Subfield: Parasitology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,801

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases