Work

MicroED data collection with SerialEM

Year: 2019

Type: article

Abstract: The cryoEM method Microcrystal Electron Diffraction (MicroED) involves transmission electron microscope (TEM) and electron detector working in synchrony to collect electron diffraction data by continuous rotation. We previously reported several protein, peptide, and small molecule structures by MicroED using manual control of the microscope and detector to collect data. Here we present a procedure to automate this process using a script developed for the popular open-source software package SerialEM. With this approach, SerialEM coordinates stage rotation, microscope operation, and camera functions for automated continuous-rotation MicroED data collection. Depending on crystal and substrate geometry, more than 300 datasets can be collected overnight in this way, facilitating high-throughput MicroED data collection for large-scale data analyses.

Source: Ultramicroscopy

DOI: 10.1016/j.ultramic.2019.03.009

Authors: M. Jason de la Cruz, Michael W. Martynowycz, Johan Hattne, Tamir Gonen

Institutions: Memorial Sloan Kettering Cancer Center, Howard Hughes Medical Institute, Janelia Research Campus, University of California, Los Angeles

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,050

Funders: Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, National Institutes of Health, National Cancer Institute