Work

Three-photon fluorescence microscopy with an axially elongated Bessel focus

Year: 2018

Type: article

Abstract: Volumetric imaging tools that are simple to adopt, flexible, and robust are in high demand in the field of neuroscience, where the ability to image neurons and their networks with high spatiotemporal resolution is essential. Using an axially elongated focus approximating a Bessel beam, in combination with two-photon fluorescence microscopy, has proven successful at such an endeavor. Here, we demonstrate three-photon fluorescence imaging with an axially extended Bessel focus. We use an axicon-based module that allowed for the generation of Bessel foci of varying numerical apertures and axial lengths, and apply this volumetric imaging tool to image mouse brain slices and for in vivo imaging of the mouse brain.

Source: Optics Letters

DOI: 10.1364/ol.43.001914

Authors: Cristina Rodríguez, Yajie Liang, Rongwen Lu, Na Ji

Institutions: Howard Hughes Medical Institute, Janelia Research Campus, University of California, Berkeley

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute of Neurological Disorders and Stroke

Awards: G2899421309, Large-scale analysis of functional synapses during circuit plasticity with novel optogenetic sensors, G9000090824, Calcium biosensors for deep-tissue imaging and spectral multiplexing