Work

Stoichiometric quantification of spatially dense assemblies with qPAINT

Year: 2019

Type: article

Abstract: Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.

Source: Nanoscale

DOI: 10.1039/c9nr00472f

Authors: Matthew A. B. Baker, Daniel J. Nieves, Geva Hilzenrat, Jonathan F. Berengut, Katharina Gaus, Lawrence K. Lee

Institutions: UNSW Sydney, Victor Chang Cardiac Research Institute

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Australian Research Council, National Health and Medical Research Council

Awards: Artificial synthesis of bacteria's molecular syringe. The type III secretion system, Artificial synthesis of the type III secretion system translocon. A new approach to vaccine design, Artificially building the bacterial flagellar motor