Year: 2019
Type: article
Abstract: Meniscal tears have a poor healing capacity, and damage to the meniscus is associated with significant pain, disability, and progressive degenerative changes in the knee joint that lead to osteoarthritis. Therefore, strategies to promote meniscus repair and improve meniscus function are needed. The objective of this study was to generate porcine meniscus-derived matrix (MDM) scaffolds and test their effectiveness in promoting meniscus repair via migration of endogenous meniscus cells from the surrounding meniscus or exogenously seeded human bone marrow-derived mesenchymal stem cells (MSCs). Both endogenous meniscal cells and MSCs infiltrated the MDM scaffolds. In the absence of exogenous cells, the 8% MDM scaffolds promoted the integrative repair of an in vitro meniscal defect. Dehydrothermal crosslinking and concentration of the MDM influenced the biochemical content and shear strength of repair, demonstrating that the MDM can be tailored to promote tissue repair. These findings indicate that native meniscus cells can enhance meniscus healing if a scaffold is provided that promotes cellular infiltration and tissue growth. The high affinity of cells for the MDM and the ability to remodel the scaffold reveals the potential of MDM to integrate with native meniscal tissue to promote long-term repair without necessarily requiring exogenous cells.
Source: Scientific Reports
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,790
Funders: U.S. Department of Health and Human Services, U.S. Department of Veterans Affairs, Arthritis Foundation, Orthopaedic Research and Education Foundation, Musculoskeletal Transplant Foundation, National Institutes of Health, National Institute on Aging, National Institute of Arthritis and Musculoskeletal and Skin Diseases
Awards: G1207333234, G1781021380, G1979292146, G2965732539, G383961551, Obesity, Biomechanics, and Inflammation in Osteoarthritis, G5175870075, Intrinsic Repair of the Knee Meniscus, G5367714306, G6317634414, Viscoelastic Properties of Normal and OA Chondrons, Weight loss, in vivo cartilage mechanics, and joint health, Mechanotransduction in Meniscus Health and Repair, In Vivo Knee Motion and ACL Biomechanics During Dynamic Activities, G8690406102, G8974702059, G9000308115, G9000336666, Washington University Rheumatic Diseases Research Resource-based Center, Duke Claude D. Pepper Older Americans Independence Center
OpenAlex ID: W2950894316