Work

A mus-51 RIP allele for transformation of Neurospora crassa

Year: 2016

Type: article

Abstract: This report describes the construction and characterization of mus-51RIP70, an allele for high-efficiency targeted integration of transgenes into the genome of the model eukaryote Neurospora crassa. Two of the mus-51RIP70 strains investigated in this work (RZS27.10 and RZS27.18) can be obtained from the Fungal Genetics Stock Center. The two deposited strains are, to our knowledge, genetically identical and neither one is preferred over the other for use in Neurospora research.

Source: Fungal Genetics Reports

DOI: 10.4148/1941-4765.1001

Authors: Zachary J. Smith, Stacy Bedore, Stephanie Spingler, Thomas M. Hammond

Institution: Illinois State University

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Institute of Child Health and Human Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Award: Identifying and characterizing proteins that detect unpaired DNA during meiosis.