Regulation of feeding behavior and hypothalamic neuropeptide gene expression by melanocortin analogues
Year: 2002
Type: dissertation
Abstract: Melanocortins are known to be involved in the inhibition of food-intake and energy metabolism.Intracerebroventricular(I.C.V) administration of several different analogues of α-MSH , such as α-MSH, NDP-MSH, α-MSH-ND, [Gln 6 ] α-MSH-ND, [Lys 6 ] α-MSH-ND, which were substituted in the position of His 6 with Gln and Lys, and Cyclic 16k-MSH to C57J/BL6 mice showed that significant inhibition of time course food-intake compared to saline-administerd control.However, truncated form of [Gln 6 ] α-MSH-ND had no effect on inhibition of food-intake.In situ hybridization and RT-PCR analysis revealed that e xpression of MCH was significantly decreased by α-MSH, NDP-MSH, α-MSH-ND, [Gln 6 ] α-MSH, [Lys 6 ] α-MSH and Cyclic 16k-MSH after 1 and 3hr of administration and e xpression of AGRP and NPY in hypothalamus was significantly decreased by α-MSH, [Gln 6 ] α-MSH, [Lys 6 ] α-MSH and Cyclic 16k-MSH after 3 hr of administration.Administration of NDP-MSH and α-MSH-ND induced a biphasic regulation in expression of AGRP and NPY, showing a decrease after 1hr and an increase after 3hr.Our results suggest that MC3R and MC4R melanocortin receptors mediate hypophagic signaling in association with differential regulation of hypothalamic neuropeptide.Identification of potential target genes in this regulation are currently undertaking to understand the signaling mediated by MC3R and MC4R in the feeding circuitry.
Author: 김병진
Language: English
Subfield: Endocrine and Autonomic Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2951244587