Molecular, Morphological, and Functional Characterization of Corticotropin-Releasing Factor Receptor 1-Expressing Neurons in the Central Nucleus of the Amygdala
Year: 2019
Type: article
Abstract: The central nucleus of the amygdala (CeA) is a brain region implicated in anxiety, stress-related disorders and the reinforcing effects of drugs of abuse. Corticotropin-releasing factor (CRF,Crh)acting at cognate type 1 receptors (CRF1,Crhr1) modulates inhibitory and excitatory synaptic transmission in the CeA. Here, we used CRF1:GFP reporter mice to characterize the morphological, neurochemical and electrophysiological properties of CRF1-expressing (CRF1+) and CRF1-non-expressing (CRF1–) neurons in the CeA. We assessed these two neuronal populations for distinctions in the expression of GABAergic subpopulation markers and neuropeptides, dendritic spine density and morphology, and excitatory transmission. We observed that CeA CRF1+ neurons are GABAergic but do not segregate with calbindin (CB), calretinin (CR), parvalbumin (PV), or protein kinase C-δ (PKCδ). Among the neuropeptides analyzed,PenkandSsthad the highest percentage of co-expression withCrhr1in both the medial and lateral CeA subdivisions. Additionally, CeA CRF1+ neurons had a lower density of dendritic spines, which was offset by a higher proportion of mature spines compared to neighboring CRF1– neurons. Accordingly, there was no difference in basal spontaneous glutamatergic transmission between the two populations. Application of CRF increased overall vesicular glutamate release onto both CRF1+ and CRF1– neurons and does not affect amplitude or kinetics of EPSCs in either population. These novel data highlight important differences in the neurochemical make-up and morphology of CRF1+ compared to CRF1– neurons, which may have important implications for the transduction of CRF signaling in the CeA.
Source: eNeuro
Institution: Scripps Research Institute
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,925
Awards: G1902745206, G194875419, Neuroplasticity of the Extended Amygdala CRF circuitry in alcohol dependence, G2002922973, G2593618960, G2845217659, G2857307434, Cross sensitization of diet and alcohol on binge behaviors and metabolic dysfunction, G3807529768, G4234095926, G56130422, G5861251585, G7269184192, Activation of the parasubthalamic nucleus in alcohol dependence, G7541045451, G8138391482, Gene-environment interaction: the brain CRF system in alcohol preferring msP rats, G9000055871, G9000083576, G9000094387, G9000107746, G9000243559, G9000264711, 9/10 Electrophysiology of Alcohol in Extended Amygdala, NEUROPSYCHOPHARMACOLOGY: MULTIDISCIPLINARY TRAINING, Gene-environment interaction: the brain CRF system in alcohol preferring msP rats, CNS Effects of Alcohol: Cellular Neurobiology
OpenAlex ID: W2951483376