Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts
Year: 2018
Type: article
Abstract: RNA is a critical component of chromatin in eukaryotes, both as a product of transcription, and as an essential constituent of ribonucleoprotein complexes that regulate both local and global chromatin states. Here we present a proximity ligation and sequencing method called Ch romatin- A ssociated R NA seq uencing (ChAR-seq) that maps all RNA-to-DNA contacts across the genome. ChAR-seq provides unbiased, de novo identification of targets of chromatin-bound RNAs including nascent transcripts, chromosome-specific dosage compensation ncRNAs, and genome-wide trans-associated RNAs involved in co-transcriptional RNA processing.
Source: eLife
DOI: 10.7554/elife.27024
Institution: Stanford University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
Funders: National Science Foundation, Howard Hughes Medical Institute, Stanford University, National Institutes of Health, School of Medicine, Stanford University, National Human Genome Research Institute, National Institute of General Medical Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: G1694074888, G1712090384, G2974218677, G3549828030, G3595593463, Determining the dynamic mechanisms regulating the maternal-to-zygotic transition, G3995718318, Cellular and Molecular Biology Training Program, Drosophila Genomics Resource Center, G443678685, G5097059376, G5653596491, Systems Biology of Collective Cell Decisions, G6382129581, G6781161026, G9000013904, G9000037719, G9000111473, G9000143808, G9000154904, G9000163467, G9000171441, G9000209201, G9000247712, G9000288206, G9000308499, G9000346909, G9000348056, Institutional Training Grant in Genome Science
OpenAlex ID: W2951525917