Enhanced Neuronal Regeneration in the CAST/Ei Mouse Strain Is Linked to Expression of Differentiation Markers after Injury
Year: 2017
Type: article
Abstract: Peripheral nerve regeneration after injury requires a broad program of transcriptional changes. We investigated the basis for the enhanced nerve regenerative capacity of the CAST/Ei mouse strain relative to C57BL/6 mice. RNA sequencing of dorsal root ganglia (DRG) showed a CAST/Ei-specific upregulation of Ascl1 after injury. Ascl1 overexpression in DRG neurons of C57BL/6 mice enhanced their neurite outgrowth. Ascl1 is regulated by miR-7048-3p, which is downregulated in CAST/Ei mice. Inhibition of miR-7048-3p enhances neurite outgrowth. Following injury, CAST/Ei neurons largely retained their mature neuronal profile as determined by single-cell RNA- seq, whereas the C57BL/6 neurons acquired an immature profile. These findings suggest that one facet of the enhanced regenerative phenotype is preservation of neuronal identity in response to injury.
Source: Cell Reports
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,620
Funders: Dr. Miriam and Sheldon G. Adelson Medical Research Foundation, National Institutes of Health, Canadian Institutes of Health Research, National Institute of Neurological Disorders and Stroke, Eunice Kennedy Shriver National Institute of Child Health and Human Development, California NanoSystems Institute
Awards: Spectral Laser Scanning Biological Microscope, Defining the molecular signature of regeneration using single cell sequencing of Macaca Mulatta dorsal root ganglia neurons, G4307096165, Boston Intellectual and Developmental Disabilities Research Center, The mechanisms of action of T-lymphocytes in neuropathic pain, Intellectual and Developmental Disablities Research Center
OpenAlex ID: W2727920467