Work

Effects of intermittent T-cell cluster disaggregation on proliferative capacity and checkpoint marker expression

Year: 2019

Type: article

Abstract: Background/aim: T-cell immunotherapies are rapidly gaining grounds in clinical success. Presently, there is first-to-market knowledge on the translation of research scale methods to clinical and commercial scales. Improved understanding can lead to more consistent and efficient production, scaling, and eventual potency. T-cell checkpoint markers, proliferation, and T-cell cluster size and disaggregation are one set of parameters that have yet to be explored.Methods: We herein activated T-cells and assessed various mechanical dissociation frequencies in relation to expression of checkpoint markers (measured by flow cytometry).Results: We herein find increased T-cell proliferation capacity with increased dissociation frequency. We also find that with increased cluster size and duration, lower proliferation, and increased expression of checkpoint markers.Conclusions: These findings reveal new translation findings with respect to T-cell handling and production and suggest that T-cell disaggregation may be important to improved cell yields and phenotype.

Source: Autoimmunity

DOI: 10.1080/08916934.2019.1630064

Authors: Matthew Li, Ling-Yee Chin, Sykuri Shukor, Alfred G. Tamayo, Marcela V. Maus, Biju Parekkadan