Year: 2019
Type: article
Abstract: SPECTROMETRY.Determination of two drugs of abuse in saliva has been performed by Paper Spray Ionization Mass Spectrometry (PSI-MS) using a Molecularly Imprinted Polymer (MIP) coated paper as substrate.The method has been optimized for quantification of 3,4-methylenedioxy-N-methylamphetamine (MDMA) and methamphetamine, 3,4-methylenedioxyamphetamine (MDA) from synthetic saliva samples.Rapid analysis (~ 1 min) of designer drugs in saliva is demonstrated using MIP-PSI-MS.Both precursor ions of MDMA and MDA were confirmed by tandem mass spectrometry (MS/MS) experiments by elucidating the fragmentation patterns formed via collisioninduced dissociation (CID).The MIP-PSI-MS method showed greater selectivity for MDMA and MDA in comparison with other analytes.Signal intensity ratios of the most important transitions for MDMA (194 → 163) and MDA (180 → 163) were found linear (R > 0,99) in the range 25-500 µg L -1 .The LOD and LOQ for MDMA were 1.11 µg L -1 and 18.73 µg L -1 , respectively, and for MDA were 3.42 µg L -1 and 21.66 µg L -1 , respectively.The precision and accuracy values were determined below 4%, and the recoveries about 100%, without pre-concentration/enrichment or separation steps.The MIP-PSI-MS method displays the potential for rapid screening for selective quantitative determination of illicit drugs in saliva samples.
Source: Química Nova
Institution: Universidade Federal de Goiás
Language: Portuguese
Subfield: Toxicology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $330
OpenAlex ID: W2953916271