Work

Low-Background Acyl-Biotinyl Exchange Largely Eliminates the Coisolation of Non- S -Acylated Proteins and Enables Deep S -Acylproteomic Analysis

Year: 2019

Type: article

Abstract: ABSTRACT Protein S -acylation (also called palmitoylation) is a common post-translational modification whose deregulation plays a key role in the pathogenesis of many diseases. Acyl-biotinyl exchange (ABE), a widely used method for the enrichment of S -acylated proteins, has the potential of capturing the entire S -acylproteome in any types of biological samples. Here, we showed that current ABE methods suffer from high background arising from the co-isolation of non- S -acylated proteins. The background can be substantially reduced by an additional blockage of residual free cysteine residues with 2,2’-dithiodipyridine prior to biotin-HPDP reaction. Coupling the low-background ABE (LB-ABE) method with label-free quantitative proteomics, 2,895 high-confidence candidate S -acylated proteins (including 1,591 known S -acylated proteins) were identified from human prostate cancer LNCaP cells, representing so-far the largest S -acylproteome dataset identified in a single study. Immunoblotting analysis confirmed the S -acylation of five known and five novel prostate cancer-related S -acylated proteins in LNCaP cells and suggested that their S -acylation levels were about 0.6-1.8%. In summary, the LB-ABE method largely eliminates the co-isolation of non- S -acylated proteins and enables deep S -acylproteomic analysis. It is expected to facilitate much more comprehensive and accurate quantification of S -acylproteomes than previous ABE methods.

Source: Analytical Chemistry

DOI: 10.1021/acs.analchem.9b01520