Bacterial quorum‐sensing signal IQS induces host cell apoptosis by targeting POT1–p53 signalling pathway
Year: 2019
Type: article
Abstract: Pseudomonas aeruginosa, an opportunistic life-threatening human bacterial pathogen, employs quorum-sensing (QS) signal molecules to modulate virulence gene expression. 2-(2-hydroxyphenyl)-thiazole-4-carbaldehyde (IQS) is a recently identified QS signal that integrates the canonical lasR-type QS of P. aeruginosa and host phosphate stress response to fine-tune its virulence production for a successful infection. To address the role of IQS in pathogen-host interaction, we here present that IQS inhibits host cell growth and stimulates apoptosis in a dosage-dependent manner. By downregulating the telomere-protecting protein POT1 in host cells, IQS activates CHK1, CHK2, and p53 in an Ataxia telangiectasia mutated (ATM)/ATM and RAD3-related (ATR)-dependent manner and induces DNA damage response. Overexpression of POT1 in host cells presents a resistance to IQS treatment. These results suggest a pivotal role of IQS in host apoptosis, highlighting the complexity of pathogenesis mechanisms developed by P. aeruginosa during infection.
Source: Cellular Microbiology
DOI: 10.1111/cmi.13076
Authors: Jianhe Wang, Chao Wang, Hong‐Bing Yu, Stephen Dela Ahator, Xiaoyan Wu, Shumei Lv, Lian‐Hui Zhang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Funders: Agency for Science, Technology and Research, Guangzhou Science and Technology Program key projects, Institute of Molecular and Cell Biology, National Key Research and Development Program of China, Biomedical Research Council, National Natural Science Foundation of China-Shandong Joint Fund for Marine Science Research Centers
Awards: G1182905213, G2366392300, G810091219
OpenAlex ID: W2954960020