Work

Genetic engineering approaches to improve posttranslational modification of biopharmaceuticals in different production platforms

Year: 2019

Type: review

Abstract: The number of approved biopharmaceuticals, where product quality attributes remain of major importance, is increasing steadily. Within the available variety of expression hosts, the production of biopharmaceuticals faces diverse limitations with respect to posttranslational modifications (PTM), while different biopharmaceuticals demand different forms and specifications of PTMs for proper functionality. With the growing toolbox of genetic engineering technologies, it is now possible to address general as well as host- or biopharmaceutical-specific product quality obstacles. In this review, we present diverse expression systems derived from mammalians, bacteria, yeast, plants, and insects as well as available genetic engineering tools. We focus on genes for knockout/knockdown and overexpression for meaningful approaches to improve biopharmaceutical PTMs and discuss their applicability as well as future trends in the field.

Source: Biotechnology and Bioengineering

DOI: 10.1002/bit.27101

Authors: Thomas Amann, Valerie Schmieder, Helene Faustrup Kildegaard, Nicole Borth, Mikael Rørdam Andersen

Institutions: Novo Nordisk Foundation, Technical University of Denmark, Austrian Centre of Industrial Biotechnology (Austria), BOKU University

Language: English

Topic: Viral Infectious Diseases and Gene Expression in Insects

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: European Commission, Novo Nordisk Fonden

Awards: Enhancing CHO by Mammalian Systems Biotechnology (eCHO systems), G7701966108