Differential gene expression and morphological changes in rat soleus muscle during the lengthened immobilization
Year: 2006
Type: dissertation
Abstract: translocase of outer membrane 22 (TOM22), solute carrier family 25 (mitochondrial adenine nucleotide translocator) member 4 (Slc25a4), cytosolic inhibitor for Nrf2 (INrf2), histone deacetylase 6 (Hdac6), αtropomyosin (Tpm1), and synaptic vesicle glycoprotein 2B (Sv2b) genes were increasingly expressed, while podocalyxin (Podx1) and Surfeit 1 (Surf1) genes were decreasingly expressed.We also found that myosin heavy chain 2B (Myh2b or Myh4) and acylphosphatase 2 (Acyp2) genes were increasingly expressed only 1 h and 1 d stretched muscle respectively.Thus, muscle stretching induced morphological changes and differential gene expression.Of 11 known genes, Atp5g3, Slc25a4 and Surf1 were related to energy metabolism; Hdac6 related to chromatin condensation; INrf2 related to homeostasis interruption; and Tpm1 and Mhy2b were contraction apparatus.Function of other genes as follows TOM22, Sv2b, Podx1, and Acyp2 are obscure.Especially, genes related to energy metabolism and chromatin condensation consider occurring apoptotic changes in mitochondria after 1 h or 1 d stretching.In EM studies swelling and cristae disruption of mitochondria support these apoptotic changes.Tpm1 gene related to the shift to fast-twitch fibers accumulated at MTJ by 4 d and 7 d stretching.Further more studies need to elucidate whether up-regulation of Tpm1 shows the shift of muscle fiber or apoptotic changes as previous results.This current study shows a better understanding of the molecular mechanism and morphological changes after muscle stretching.
Author: 김지원
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2955093251