Work

Effects of Sucrose and Browning Inhibitors on Callus Proliferation and Anti-Browning of Chinese Kale

Year: 2019

Type: conference-paper

Abstract: The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants, the effects of the different concentrations of sucrose and different browning inhibitors (activated carbon, polyvinylpyrrolidone, and ascorbic acid) on the callus proliferation and anti-browning of Chinese kale were studied in this study. The results showed that the proliferation medium with 20 g·L −1 sucrose had the best effects on the proliferation and anti-browning of Chinese kale callus, and its proliferation rate was the highest, reaching 213.5%, and the browning rate was as low as 27.77%. In addition, adding 0.2 g·L −1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate. In this treatment, the callus had the lowest browning rate of 19.45% and the highest proliferation rate of 266.98%, and the morphology of the callus was friable. This study lays a foundation for future research in molecular biology and genetic improvement in Chinese kale.

Source: IOP Conference Series Earth and Environmental Science

DOI: 10.1088/1755-1315/252/2/022018

Authors: Sha Liang, Ying He, Hao Zheng, Qiao Yuan, Fen Zhang, Bo Sun

Institution: Sichuan Agricultural University

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

Funders: National Natural Science Foundation of China, Sichuan Agricultural University