Work

Novel method of single antibiotic selection of cells containing multiple stage-specific promoter-fluorophore plasmids.

Year: 2010

Type: article

Abstract: This study demonstrates a novel method by which multiple separate plasmids can be stably integrated into the genome using single antibiotic resistance for selection. This method was used to integrate three different cardiac-specific promoters driving different fluorophores into murine embryonic stem cells, allowing sequential visualization of various stages of cardiac differentiation. This method is broadly applicable to the study of cell lineage of different stem/progenitor cells.

Source: PubMed

Authors: Achuta K Guddati, John A Kessler

Institutions: Harlem Hospital Center, Columbia University

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institutes of Health

Awards: Regulation of Neurotransmitter Metabolism, Mechanisms Governing Neuronal Development