Work

Artificial Metalloenzymes: Challenges and Opportunities

Year: 2019

Type: review

Abstract: Artificial metalloenzymes (ArMs) result from the incorporation of an abiotic metal cofactor within a protein scaffold. From the earliest techniques of transition metals adsorbed on silk fibers, the field of ArMs has expanded dramatically over the past 60 years to encompass a range of reaction classes and inspired approaches: Assembly of the ArMs has taken multiple forms with both covalent and supramolecular anchoring strategies, while the scaffolds have been intuitively selected and evolved, repurposed, or designed in silico. Herein, we discuss some of the most prominent recent examples of ArMs to highlight the challenges and opportunities presented by the field.

Source: ACS Central Science

DOI: 10.1021/acscentsci.9b00397

Authors: Holly J. Davis, Thomas R. Ward

Institution: University of Basel

Language: English

Topic: Metal-Catalyzed Oxygenation Mechanisms

Subfield: Inorganic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: diamond

Funders: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, H2020 European Research Council

Awards: Directed Evolution of Artificial Metalloenzymes for In Vivo Applications, Directed Evolution of Artificial Metalloproteins and Metalloenzymes