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RNase H–dependent PCR-enabled T-cell receptor sequencing for highly specific and efficient targeted sequencing of T-cell receptor mRNA for single-cell and repertoire analysis

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Year: 2019
Type: article
Source: Nature Protocols
DOI: 10.1038/s41596-019-0195-x
Authors: Shuqiang Li, Jing Sun, Rosa Allesøe, Krishnalekha Datta, Yun Bao, Giacomo Oliveira, Juliet Forman, Roger Jin, Lars Rønn Olsen, Derin B. Keskin, Sachet A. Shukla, Catherine J. Wu, Kenneth J. Livak
Institutions: Broad Institute, Dana-Farber Cancer Institute, Technical University of Denmark, Integrated DNA Technologies (United States), Massachusetts Institute of Technology
Language: English

Topic: Single-cell and spatial transcriptomics
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
SDG: Good health and well-being

Open Access status: green

Funder: National Cancer Institute
Awards: G1066262359, G1273380608, G1791404784, G3433906362, G44360338, G6644283022, Immune Modulation After Allogeneic HCT
OpenAlex ID: W2962729112
FWCI: 1.217
Cites: 52
Cited by: 41
Related to: 10
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