Work

Nonsense‐mediated mRNA decay of the ferric and cupric reductase mRNA s FRE 1 and FRE 2 in Saccharomyces cerevisiae

Year: 2019

Type: article

Abstract: The nonsense-mediated mRNA decay (NMD) pathway regulates mRNAs that aberrantly terminate translation. This includes aberrant mRNAs and functional natural mRNAs. Natural mRNA degradation by NMD is triggered by mRNA features and environmental cues. Saccharomyces cerevisiae encodes multiple proteins with ferric and cupric reductase activity. Here, we examined the regulation by NMD of two mRNAs, FRE1 and FRE2, encoding ferric and cupric reductases in S. cerevisiae. We found that FRE2 mRNAs are regulated by NMD under noninducing conditions and that the FRE2 3'-UTR contributes to the degradation of the mRNAs by NMD. Conversely, FRE1 mRNAs are not regulated by NMD under comparable conditions. These findings suggest that regulation of functionally related mRNAs by NMD can be differential and conditional.

Source: FEBS Letters

DOI: 10.1002/1873-3468.13545

Authors: Megan Peccarelli, Taylor D. Scott, Bessie W. Kebaara

Institution: Baylor University

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Texas Higher Education Coordinating Board, Baylor University, National Institutes of Health, National Institute of General Medical Sciences

Award: Investigation of copper homeostasis mRNAs regulated by the Nonsense-mediated mRNA decay pathway