Work

Microrheological quantification of viscoelastic properties with photonic force optical coherence elastography

Year: 2019

Type: article

Abstract: Photonic force optical coherence elastography (PF-OCE) is a new approach for volumetric characterization of microscopic mechanical properties of three-dimensional viscoelastic medium. It is based on measurements of the complex mechanical response of embedded micro-beads to harmonically modulated radiation-pressure force from a weakly-focused beam. Here, we utilize the Generalized Stokes-Einstein relation to reconstruct local complex shear modulus in polyacrylamide gels by combining PF-OCE measurements of bead mechanical responses and experimentally measured depth-resolved radiation-pressure force profile of our forcing beam. Data exclusion criteria for quantitative PF-OCE based on three noise-related parameters were identified from the analysis of measurement noise at key processing steps. Shear storage modulus measured by quantitative PF-OCE was found to be in good agreement with standard shear rheometry, whereas shear loss modulus was in agreement with previously published atomic force microscopy results. The analysis and results presented here may serve to inform practical, application-specific implementations of PF-OCE, and establish the technique as a viable tool for quantitative mechanical microscopy.

Source: Optics Express

DOI: 10.1364/oe.27.022615

Authors: Nichaluk Leartprapun, Yuechuan Lin, Steven G. Adie

Language: English

Topic: Optical Coherence Tomography Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funder: National Institute of Biomedical Imaging and Bioengineering

Awards: G2307581443, Volumetric Traction Force Tomography of Collective Cell Migration Dynamics, THREE-DIMENSIONAL MECHANO-MICROSCOPY OF THE STEM CELL NICHE, G7661121960