Effect of Cultivars, Auxins and Activated Charcoal on in Vitro Roots Formation of Strawberry Plantlets
Year: 2019
Type: article
Abstract: This experiment amid to investigate the effect of two strawberry cultivars (Festivaland Sweet Charlie), two auxins, i.e. Indole-3-butyric acid (IBA) and1-Naphthalene acetic acid (NAA)and activated charcoal on the in vitro roots formation (rooting stage) of strawberry plantlets. Theobtained results showed that, Sweet Charlie cultivar being the superior one and recorded the maximumvalues of both root and shoot length, number of roots and leaves, the fresh and dry weight of roots andleaves of plantlet as compared with Festival cultivar. It is evident that, using ½ MS-mediumsupplemented with 1.0 mg /l IBA+0.5 g /l activated charcoal being the most effective and superiortreatment for increasing both root and shoot length. While, rooting medium contained ½ MS-medium+ 1 .0 mg /l IBA or 0.5mg /l IBA +0.5 mg /l NAA recorded the highest values of number of both rootsand leaves per plantlet .In addition ,using ½ MS-medium supplemented with 1.0 mg /l NAA recordedthe maximum values of the fresh and dry weight of roots per plantlet. Furthermore, the highest valuefor each of fresh weight of crown and leaves per plantlet, was obtained by using ½ MS-mediumsupplemented with 0.5 mg /l IBA+0.5 mg /l NAA. On the other hand, using ½ MS–medium + 0.5 mg/l IBA + 0.5 mg /l NAA + 0.5g /l activated charcoal being the superior treatment and recorded themaximum values of nitrogen, phosphorus, potassium, total carbohydrate and total protein content (%).Transfer shoots of Sweet Charlie cultivar to the rooting medium contained ½ MS- medium +1.0 mg /lIBA +0.5 g/l activated charcoal recorded the maximum values of both root and shoot length, numberof leaves per plantlet, the fresh and dry weight of leaves, as well as the dry weight of crown perplantlet. While, the highest number of roots per plantlet was more achieve via using ½ MS-medium +1.0 mg/l IBA. Moreover, cultured shoots of Festival cultivar on ½ MS-medium supplemented with 0.5mg /l IBA+0.5 mg /l NAA being the most effective and favorable treatment for increasing the freshweight of roots per plantlet. On the other hand, the highest increase of the fresh weight of crown perplantlet were more distinct via the interaction treatment between such cultivar and using ½ MSmediumsupplemented with 1.0 mg/l NAA. Furthermore, using the rooting culture medium contained½ MS- medium+0.5 mg/l IBA+0.5 g /l activated charcoal recorded the highest value for each ofnitrogen and total protein content (%) in the plantlets of Festival cultivar, while cultured shoots ofSweet Charlie cultivar on the same rooting medium, registered the maximum increase of phosphorus,potassium and total carbohydrates content (%).
Institutions: Agricultural Research Center, Zagazig University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2964577526