Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
Year: 2019
Type: article
Abstract: Particles of diamond in the 5-100 nm size range, known as nanodiamond (ND), have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photostability, they are not only suitable for two-photon imaging, but also allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of ND can also be of use when characterizing adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.
Source: Royal Society Open Science
DOI: 10.1098/rsos.190589
Institution: University of Strathclyde
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,471
Awards: G1727918786, G2041887790, G2045692093, EPSRC-Royal Society fellowship engagement (2013):Visualising thoughts: ESR measurement of nanodiamonds targetted to neural structures, G5290901136, G6879686769, EPSRC-Royal Society fellowship engagement (2013):Visualising thoughts: ESR measurement of nanodiamonds targetted to neural structures
OpenAlex ID: W2964995389