Integrated microfluidic systems with sample preparation and nucleic acid amplification
Year: 2019
Type: review
Abstract: Rapid, efficient and accurate nucleic acid molecule detection is important in the screening of diseases and pathogens, yet remains a limiting factor at point of care (POC) treatment. Microfluidic systems are characterized by fast, integrated, miniaturized features which provide an effective platform for qualitative and quantitative detection of nucleic acid molecules. The nucleic acid detection process mainly includes sample preparation and target molecule amplification. Given the advancements in theoretical research and technological innovations to date, nucleic acid extraction and amplification integrated with microfluidic systems has advanced rapidly. The primary goal of this review is to outline current approaches used for nucleic acid detection in the context of microfluidic systems. The secondary goal is to identify new approaches that will help shape future trends at the intersection of nucleic acid detection and microfluidics, particularly with regard to increasing disease and pathogen detection for improved diagnosis and treatment.
Source: Lab on a Chip
DOI: 10.1039/c9lc00389d
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
Funders: National Science Foundation, State Key Laboratory of Industrial Control Technology, National Institutes of Health, National Key Research and Development Program of China, Fundamental Research Funds for the Central Universities, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke
OpenAlex ID: W2966056094