Work

Chemotaxis based enrichment for transgenic animals containing the rol-6 marker

Year: 2018

Type: article

Abstract: Utilizing the chemotaxis of C. elegans, we can separate wild-type C. elegans from “rollers.” If a mixed population of transgenic worms harboring the rol-6 marker, and their non-transgenic siblings are plated a distance from food, “rollers” will be unable to efficiently migrate towards the food due to impaired locomotion, while non-transgenic C. elegans will have normal locomotion and be able to quickly migrate to the food source. This will efficiently separate the two types of worms and will allow for the isolation of a mostly transgenic population.

Source: PubMed Central

DOI: 10.17912/kedf-yn42

Authors: Carter, John L., Morales, Rafael, Johnson, Steven M.

Institution: Brigham Young University

Language: English

Topic: Animal Genetics and Reproduction

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: Overcoming Transgene Silencing by DNA-Directed Chromatin Reconformation