Year: 2019
Type: article
Abstract: Most microarray image scanning approaches provide an estimation of the intensity of the foreground and background for each spot. Background intensity must be corrected in order to remove the effect of non-specific binding or spatial heterogeneity across the array, but when such corrections are applied many problems appear, such as negative intensity for the spot or high variability of low-intensity log ratios. In this paper, many alternative methods for calculating background intensity are discussed and many approaches for background correction are tested and compared. GenePix, ScanAlyze and QuantArry are the strategies that were reviewed for background locations to extract their intensity. Similarly, to GenePix, a new approach for background calculation was proposed and tested. It shows more accurate results and the occurrences of error become lesser.
Authors: Omar Salem Baans, Asral Bahari Jambek
Institution: Universiti Malaysia Perlis
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2967957452