Integrated Single-Cell Transcriptomics and Chromatin Accessibility Analysis Reveals Regulators of Mammary Epithelial Cell Identity
Year: 2020
Type: article
Abstract: The mammary epithelial cell (MEC) system is a bilayered ductal epithelium of luminal and basal cells, maintained by a lineage of stem and progenitor populations. Here, we used integrated single-cell transcriptomics and chromatin accessibility analysis to reconstruct the cell types of the mouse MEC system and their underlying gene regulatory features in an unbiased manner. We define differentiation states within the secretory type of luminal cells, which forms a continuous spectrum of general luminal progenitor and lactation-committed progenitor cells. By integrating single-cell transcriptomics and chromatin accessibility landscapes, we identify cis- and trans-regulatory elements that are differentially activated in the specific epithelial cell types and our newly defined luminal differentiation states. Our work provides a resource to reveal cis/trans-regulatory elements associated with MEC identity and differentiation that will serve as a reference to determine how the chromatin accessibility landscape changes during breast cancer.
Source: Cell Reports
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,200
Funders: American Cancer Society, Chan Zuckerberg Initiative, National Institutes of Health, Japan Society for the Promotion of Science, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Office of Extramural Research, National Institutes of Health
Awards: G104966582, G1443287152, Proteolytic regulation of Wnt signaling in breast cancer stem cells, G2250921843, Microenvironmental regulation of breast tumorigenesis, G2686076696, Mammary basal/stem cell plasticity and regulation, G4482581187, Multiscale Models of Wound Cell Plasticity for Regeneration
OpenAlex ID: W2968690185