Work

Ex vivo electrochemical measurement of glutamate release during spinal cord injury

Year: 2019

Type: article

Abstract: Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from ex vivo rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors.

Source: MethodsX

DOI: 10.1016/j.mex.2019.08.008

Authors: James K. Nolan, Tran N.H. Nguyen, Mara Fattah, Jessica C. Page, Riyi Shi, Hyowon Lee

Institution: Purdue University West Lafayette

Language: English

Topic: Spinal Cord Injury Research

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $600