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Comparison of Three Adenovirus Quantitative PCR Assays with ATCC Reference Strains and Clinical Samples

Year: 2019

Type: article

Abstract: ) for all culture fluids tested. All three assays resulted in endpoint detection of the available 51 AdV types. Using two different materials to generate a standard curve revealed that the Octaplex TaqMan assay and the modified Jothikumar assay both consistently gave adenovirus levels lower than the commercial platform for AdV culture fluids but not patient samples. This study highlights the differences in detection of AdV between laboratories that can be attributed to both the PCR method, as well as the reference material used for quantitation.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.00735-19

Authors: Kimberly Starr, Alexander L. Greninger, Negar Makhsous, Keith R. Jerome, Linda Cook

Institutions: University of Washington Medical Center, Fred Hutch Cancer Center

Language: English

Topic: Respiratory viral infections research

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2969606836