Feasibility and Safety of Multicenter Tissue and Biofluid Sampling for α -Synuclein in Parkinson’s Disease: The Systemic Synuclein Sampling Study (S4)
Year: 2018
Type: article
Study design: Observational Study
Abstract: BACKGROUND: α-synuclein is a lead Parkinson's disease (PD) biomarker. There are conflicting reports regarding accuracy of α-synuclein in different tissues and biofluids as a PD biomarker, and the within-subject anatomical distribution of α-synuclein is not well described. The Systemic Synuclein Sampling Study (S4) aims to address these gaps in knowledge. The S4 is a multicenter, cross-sectional, observational study evaluating α-synuclein in multiple tissues and biofluids in PD and healthy controls (HC). OBJECTIVE: To describe the baseline characteristics of the S4 cohort and safety and feasibility of this study. METHODS: Participants underwent motor and non-motor clinical assessments, dopamine transporter SPECT, biofluid collection (cerebrospinal fluid, saliva, and blood), and tissue biopsies (skin, sigmoid colon, and submandibular gland). Biopsy adequacy was determined based on presence of adequate target tissue. Tissue sections were stained with the 5C12 monoclonal antibody against unmodified α-synuclein. All specimens were acquired and processed in a standardized manner. Adverse events were systematically recorded. RESULTS: The final cohort consists of 82 participants (61 PD, 21 HC). In 68 subjects (83%), all types of specimens were obtained but only 50 (61%) of subjects had all specimens both collected and evaluable for α-synuclein. Mild adverse events were common, especially for submandibular gland biopsy, but only 1 severe adverse event occurred. CONCLUSION: Multicenter tissue and biofluid sampling for α-synuclein is feasible and generally safe. S4 will inform understanding of the concurrent distribution of α-synuclein pathology and biomarkers in biofluids and peripheral nervous system in PD.
Source: Journal of Parkinson s Disease
DOI: 10.3233/jpd-181434
Authors: Lana M. Chahine, Thomas G. Beach, Nicholas Seedorff, Chelsea Caspell-Garcia, Christopher S. Coffey, Michael Brumm, Charles H. Adler, Geidy E. Serrano, Carly Linder, Sherri Mosovsky, Tatiana Foroud, Holly Riss, Dixie Ecklund, John Seibyl, Danna Jennings, Vanessa Arnedo, Lindsey Riley, K.D. Dave, Brit Mollenhauer, on behalf of the Systemic sSynuclein Sampling study
Institutions: University of Pittsburgh, Banner Sun Health Research Institute, University of Iowa, Mayo Clinic, Mayo Clinic in Arizona, University of Pennsylvania, Indiana University Indianapolis, Indiana University School of Medicine, Institute for Neurodegenerative Disorders, Denali Therapeutics (United States), Michael J. Fox Foundation, Universitätsmedizin Göttingen, Paracelsus Elena Klinik Kassel
Language: English
Subfield: Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W2889766728