A general fluorescent light-up probe for staining and quantifying protein
Year: 2019
Type: article
Abstract: Proteins are the primary functional agents in all cellular processes, facilitating various functions such as enzymes and structure-forming or signal-transducing molecules. In this work, we report a fluorescent dye, PyMDI-Zn, which could specifically bind with proteins and provide a red-shifted fluorescent emission. The visual analysis of protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis could be realized in 5 min by using PyMDI-Zn as a light-up dye. Based on its cell penetration and low toxicity, PyMDI-Zn could also be applied to locate protein-rich regions and organelles in live cell imaging. Moreover, the direct protein quantitation can be realized based on PyMDI-Zn, providing a method of screening for food adulteration by nitrogen-rich compounds.
Source: Royal Society Open Science
DOI: 10.1098/rsos.190580
Authors: Jiawei Zou, Gangyi Chen, Feng Du, Yi Yuan, Xin Huang, Juan Dong, Kexin Xie, Xin Cui, Zhuo Tang
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,471
OpenAlex ID: W2970580380