Work

Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

Year: 2019

Type: article

Abstract: Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enabling simultaneous quantification of the lesions on individual DNA molecules. We follow the repair of both damage types in skin cells exposed to artificial sunlight.

Source: Chemical Communications

DOI: 10.1039/c9cc05198h

Authors: Dmitry Torchinsky, Yael Michaeli, Natalie R. Gassman, Yuval Ebenstein

Institutions: Tel Aviv University, USA Mitchell Cancer Institute, University of South Alabama

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green