Year: 2016
Type: review
Abstract: The Protein Data Bank (PDB), created in 1971 when merely seven protein crystal structures were known, today holds over 120, 000 experimentally-determined three-dimensional models of macromolecules, including gigantic structures comprised of hundreds of thousands of atoms, such as ribosomes and viruses. Most of the deposits come from X-ray crystallography experiments, with important contributions also made by NMR spectroscopy and, recently, by the fast growing Cryo-Electron Microscopy. Although the determination of a macromolecular crystal structure is now facilitated by advanced experimental tools and by sophisticated software, it is still a highly complicated research process requiring specialized training, skill, experience and a bit of luck. Understanding the plethora of structural information provided by the PDB requires that its users (consumers) have at least a rudimentary initiation. This is the purpose of this educational overview.
Source: Postępy Biochemii
DOI: 10.18388/pb.2016_1
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: diamond
Awards: The Protein Structure Inittiative Knowledgebase, Metal binding sites in macromolecular structures, Integrated resource for reproducibility in macromolecular crystallography, G3300556294, G4336488413, Midwest Center for Structural Genomics, The Crystallography of Macromolecules, G6601593777, G7968358368, X-ray data analysis in the presence of structural variability, G8389135606
OpenAlex ID: W2970929303