Work

Molecular identification of coagulase-negative staphylococci by rpoB sequence typing

Year: 2018
Type: article
Abstract: Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM.Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies.The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections.In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing.As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.
Language: English
Open Access status: diamond
OpenAlex ID: W2971744429
FWCI:
Cites: 9
Cited by: 2
Related to: 10