Year: 2006
Type: conference-paper
Abstract: We describe the functional elements of a diagnostic instrument and disposable enteric card (DEC) system under development that rapidly identifies and differentiates Shigella dysenteriae serotype 1, Escherichia coli O157:H7, Campylobacter jejuni, and Salmonella and Shigella species in stool samples.These elements, currently realized as microfluidic subcircuits on individual cards, are (1) whole pathogen immunocapture, (2) nucleic acid extraction, (3) on-chip rapid PCR, and (4) lateral flow detection of amplicons.The system utilizes a low-cost disposable lab-on-a-card platform designed to identify enteric bacterial pathogens in patients with acute diarrhea.Special emphasis is placed on the utility of the device to diagnose both intentionally released enteric biothreat agents as well as to provide a platform to identify infections that are common worldwide.All reagents are stored in dry form on the card.In this paper we report on the design and validation of individual subcircuits, identification and validation of capture antibodies and strategy for organism immunocapture, and identification and validation of specific PCR primer sequences for over 200 clinical isolates of enteric pathogens as well as spiked and pathogenic stool samples.
Authors: B.H. Weigl, J. Gerdes, P. Tarr, P. Yager, L. Dillman, J. Gerlach, M. Steele, R. Peck, S. Ramachandran, M. Lemba, D. Hoekstra, M. Kokoris, M. Nabavi, F. Battrell, D.M. Denno, E.J. Klein
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W2971989161