Work

Solid immersion meniscus lens (SIMlens) for open-top light-sheet microscopy

Year: 2019

Type: article

Abstract: Open-top light-sheet (OTLS) microscopy has been developed for rapid volumetric imaging of large pathology specimens. A challenge with OTLS microscopy is the transmission of oblique illumination and detection beams through a horizontal sample plate without introducing aberrations. Previous solutions prevented vertical sample movement, constrained the refractive index of the sample, and/or hindered multi-resolution imaging. Here we describe a solid immersion meniscus lens, a wavefront-matching element that suppresses aberrations when illumination and detection beam transition between air and various high-index immersion media, thereby enabling multi-resolution OTLS microscopy of specimens cleared with diverse protocols.

Source: Optics Letters

DOI: 10.1364/ol.44.004451

Authors: Lindsey A. Barner, Adam K. Glaser, Lawrence D. True, Nicholas P. Reder, Jonathan T. C. Liu

Institution: University of Washington

Language: English

Topic: Advanced optical system design

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green