Work

A Nonhomologous End-Joining Mutant for Neurospora sitophila Research

Year: 2020

Type: article

Abstract: Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency of transgene integration into targeted genomic locations of Neurospora crassa and other fungi. Here, we report that a similar phenomenon occurs in a second Neurospora species: N. sitophila . Specifically, we show that deletion of N. sitophila mus-51 increases the efficiency of targeted-transgene integration, presumably by disrupting NHEJ. Researchers interested in obtaining the N. sitophila mus-51 Δ strains described in this study can obtain them from the Fungal Genetics Stock Center (FGSC).

Source: Fungal Genetics Reports

DOI: 10.4148/1941-4765.2172

Authors: Nicholas A. Rhoades, Elise K. Webber, Thomas M. Hammond

Institution: Illinois State University

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funder: National Science Foundation

Award: How do Spore killers kill spores? Elucidating the mechanism of meiotic drive by spore killing in Neurospora fungi.